ANTIBACTERIA ASSAY OF PINEAPPLE PEEL (Ananas Comosus L. MERR) MOUTHWASH EXTRACT FORMULA AGAINST Staphylococcus aureus

  • Helfi Nofita STIKES Muhammadiyah Pekajangan Pekalongan
  • Eko Mugiyanto STIKES Muhammadiyah Pekajangan Pekalongan
  • Wulan Agustiningrum STIKES Muhammadiyah Pekajangan Pekalongan

Abstract

Staphylococcus Aureus is a facultative anaerobes gram-positive bacteria that cause bad breath. Pineapple skin has enzyme bromelin, tannin, chlorine, phenol, which has the suppressing effect of bacterial growth. This aim of this study is to find out and evaluate the best pineapple extract (Ananas Comosus L. Merr) mouthwash preparation formula that meets physical requirements and has the growth inhibition of Staphylococcus Aureus ATCC 25923. Antibacterial activity assay used NA media by agar diffusion method. Physical assay include organoleptic test, pH, viscosity, and cycling test in variation of concentration extract 0,78 g, 1,56 g and 3,12 g. Antibacterial assay showed inhibitories of 1,67 mm, 7,67 mm, 11,33 mm for F1, F2, and F3 respectively with significantly different at  p < 0,05.The results show that the F3 has the bigest inhibition growth and meet the physical requirements..

References

fni N, dkk. (2015). Uji Aktivitas Antibakteri Pasta Gigi Ekstrak Biji Pinang (Areca catechu L) Terhadap Streptococus mutans Dan Staphylococus aureus. GALENIKA Journal of Pharmacy Vol. 1 (1): 48-58 ISSN: 2442-8744. Palu
Anonim(1), 1985, Cara Pembuatan Simplisia, Departemen Kesehatan Republik Indonesia,
Anonim(2), 1995. Farmakope Indonesia Edisi ke-IV. Jakarta: Departemen Kesehatan RI.
Anonim(3), 1989, Material Medika Indonesia. Jilid V, Departemen Kesehatan Republik Indonesia, Jakarta.
Anonim(4), 2000, Parameter Standar Umum Ekstrak Tumbuhan Obat, Direktorat Jendral Pengawasan Obat dan Makanan Departemen Kesehatan Republik Indonesia, Jakarta.
Cuesta Al, Jewtuchowicz V Brusca MI, Nastri ML, Rosa AC. 2010. Prevalence of Staphylococcus sppand candida spp in the oral cavity and periodontal pockets of periodontal disease patients. Acta Odontol Latinoam. 23(1): 20-6.
Davis, W.W.,Stout, T.R. 1971. Disc Plate Method Of Microbiologi Assay. Journal of microbiology. 22(4):659-665.
Harbone, J. B, 1987. Metode Fitokimia: penuntun cara modern menganalisa tumbuhan. Penerbit ITB. Bandung.
Hatam, Sri Febriani. Edi Suryono, Jemmy Abidjulu 2013. Aktivitas antioksidan dari Ekstrak Kulit Nanas (Ananas comosus. L. Merr). Program Studi Farmasi UNSRAT Manado. Jurnal Ilmiah Farmasi –UNSRAT, Vol.2, No.01,Hal.8-2.
Husna, Asmaul dan Abral, 2014. Efektivitas Obat Kumur dalam Menghilangkan Bau Mulut (halitosis) pada Perokokaktif., Jurusan Keperawatan Gigi Poltekkes. Pontianak.
Martin, A., Swarbick, J., dan A, Cammarata. 1993. Farmasi Fisik 2. Edisi III. Jakarta: UI Press. Pg. 940-1010, 1162,1163, 1170
Rachma, Muthia, 2010. Formulasi sediaan obat kumurr yang mengandung minyak atsiri temulawak (Curcuma xanthorriza) sebagai anti bakteri porphyromonas gingivalis penyebab bau mulut. Fakultas matematika dan ilmu pengetahuanalam. Program studi farmasi. Universitas Indonesia. Depok.
Rakhmanda, Adi Putra 2008. Perbandingan Efek Antibakteri Jus Nanas (Ananascomosus. L. Merr). Pada Berbagai Konsentrasi terhadap Streptococcus Mutans. Artikel Karya Tulis Ilmiah Fakultas Kedokteran Universitas Diponegoro. Semarang .
Rowe, R. C, Sheskey, P. J & Owen, S. C(2). 2009. Handbook of Pharmaceutical Excipients, 6th ed. London: The Pharmaceutical Press.
Setyowati, W.A.E., Ashadi, Ariani, S.R.D., Mulyani, B. dan Rahmawati, C.P., (2014). Skrining Fitokimia dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibenthinus Murr.) Varietas Petruk. Prosiding Seminar Nasional Kimia dan Pendidikan Kimia VI, Program Studi Pendidikan Kimia Jurusan PMIPA FKIP UNS, Surakarta.
Wilkinson, J. B., dan Moore, R.J. 1982. Harry’s Cosmeticology. Edisi ketujuh. New York: Chemical Publishing Company. Halaman 3, 231-232, 240-241, 248.
Yoshepine, Ardiana Dewi, Wulanjati Martha Purnami, Saifullah Teuku Nada, Astuti Puji. 2013. Mouthwash Formulation of Basil Oil (Ocimum basilicum L.) and In Vitro Antibacterial and Antibiofilm Activities Against Stretococcus mutans. Traditional Medicine Journal.Vol 18 (2), pg 95-102, Universitas Gadjah Mada. Yogyakarta.
Published
2018-10-07
How to Cite
NOFITA, Helfi; MUGIYANTO, Eko; AGUSTININGRUM, Wulan. ANTIBACTERIA ASSAY OF PINEAPPLE PEEL (Ananas Comosus L. MERR) MOUTHWASH EXTRACT FORMULA AGAINST Staphylococcus aureus. JCPS (Journal of Current Pharmaceutical Sciences), [S.l.], v. 2, n. 1, p. 97-103, oct. 2018. ISSN 2598-2095. Available at: <http://journal.umbjm.ac.id/index.php/jcps/article/view/158>. Date accessed: 19 oct. 2018.