The ANTIBIOTICS SENSITIVITY TEST ON Escherichia coli THAT CAUSE DIARRHEA IN MANADO CITY

  • Oksfriani Jufri Sumampouw Universitas Sam Ratulangi

Abstract

Antibiotics resistance has become an important issue in the health, especially pharmacy. Several types of bacteria have been found that have the ability to resist antibiotics. One type of bacteria was Escerichia coli (E. coli). E. coli was one of the microorganisms that cause diarrhea. The purpose of this study was to determine the sensitivity of antibiotics to E. coli causing diarrhea in the Manado city. This research was an experimental research. This research was located at the Microbiology Laboratory, Faculty of Fisheries and Marine Sciences Sam Ratulangi University. Sensitivity testing using the Well method. The results showed that at a dose of 0.1 ppm all antibiotics could inhibit bacterial growth. Resistance begins to occur at the concentration of antibiotics of 0.001 ppm. The conclusion of this study was that E. coli has the ability to resist the Chlorampenicol, Ampicillin, Amoxicillin, and Tetracyclin. In contrast to Ciprofloxacin resistance has not been found.

References

Banin, E., Hughes, D., & Kuipers, O. P. (2017). Bacterial pathogens, antibiotics and antibiotic resistance. FEMS microbiology reviews, 41(3), 450-452.
Blanco, J., Mora, A., Mamani, R., López, C., Blanco, M., Dahbi, G., ... & Pascual, Á. (2011). National survey of Escherichia coli causing extraintestinal infections reveals the spread of drug-resistant clonal groups O25b: H4-B2-ST131, O15: H1-D-ST393 and CGA-D-ST69 with high virulence gene content in Spain. Journal of antimicrobial chemotherapy, 66(9).
Centers for Disease Control and Prevention. 2014. Escherichia coli (E. coli). (Online) diakses dari http://www.cdc.gov/ecoli/general/index.html diakses pada 6 Nopember 2014
Estrada-García, T., Cerna, J. F., Paheco-Gil, L., Velázquez, R. F., Ochoa, T. J., Torres, J., & DuPont, H. L. (2005). Drug-resistant diarrheogenic Escherichia coli, Mexico. Emerging infectious diseases, 11(8), 1306.
Geisinger, E., & Isberg, R. R. (2017). Interplay between antibiotic resistance and virulence during disease promoted by multidrug-resistant bacteria. The Journal of infectious diseases, 215(suppl_1), S9-S17.
Ho, P. L., Chow, K. H., Lai, E. L., Lo, W. U., Yeung, M. K., Chan, J., ... & Yuen, K. Y. (2011). Extensive dissemination of CTX-M-producing Escherichia coli with multidrug resistance to ‘critically important’antibiotics among food animals in Hong Kong, 2008–10. Journal of antimicrobial chemotherapy, 66(4), 765-768.
Johnson, J. R., Johnston, B., Clabots, C., Kuskowski, M. A., & Castanheira, M. (2010). Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States. Clinical infectious diseases, 51(3), 286-294.
Kumarasamy, K. K., Toleman, M. A., Walsh, T. R., Bagaria, J., Butt, F., Balakrishnan, R., ... & Woodford, N. (2010). Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. The Lancet infectious diseases, 10(9), 597-602.
Li, B., & Webster, T. J. (2018). Bacteria antibiotic resistance: New challenges and opportunities for implant‐associated orthopedic infections. Journal of Orthopaedic Research®, 36(1), 22-32.
Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., ... & Monnet, D. L. (2012). Multidrug‐resistant, extensively drug‐resistant and pandrug‐resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3), 268-281.
Poirel, L., Lagrutta, E., Taylor, P., Pham, J., & Nordmann, P. (2010). Emergence of metallo-β-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia. Antimicrobial agents and chemotherapy, 54(11), 4914-4916.
Public health agency of Canada. 2014. E. coli (online) diakses dari http://www.phac-aspc.gc.ca/fs-sa/fs-fi/ecoli-eng.php pada 6 Nopember 2014Ariyani, N., & Sari, R. A. (2018). DOXYCYCLINE AND CIPROFLOXACIN RESISTANCE IN Escherichia coli ISOLATED FROM LAYER FECES.
Sack, R. B., Rahman, M., Yunus, M., & Khan, E. H. (1997). Antimicrobial resistance in organisms causing diarrheal disease. Clinical infectious diseases,24(Supplement 1), S102-S105.
Sholeh, M. A. (2018). KUANTITAS PENGGUNAAN ANTIBIOTIK DAN POLA RESISTENSI ESCHERICHIA COLI FLORA NORMAL USUS DI RUANG RAWAT INTENSIF DAN RUANG RAWAT TROPIK INFEKSI DI RSUD Dr. SOETOMO SURABAYA (Doctoral dissertation, Universitas Airlangga).
Tadesse, D. A., Zhao, S., Tong, E., Ayers, S., Singh, A., Bartholomew, M. J., & McDermott, P. F. (2012). Antimicrobial drug resistance in Escherichia coli from humans and food animals, United States, 1950-2002. Emerg. Infect. Dis, 18, 741-749.
Todar, K. 2008. Antimicrobial Agents Used in Treatment of Infectious Disease. University ofWisconsin Madison Department of Bacteriology. Available from http://www.textbookofbacteriolgy.net/antimicrobe (13 Maret 2008)
Wikipedia. 2008a. Ciprofloxacin. Wikipedia, The Free Encyclopedia. Available from http://en.wikipedia.org/wiki/Ciprofloxacin. (15 April 2008)
Wikipedia. 2008b. Tetracycline. Wikipedia, The Free Encyclopedia. Available from http://en.wikipedia.org/wiki/Tetracycline. (15 April 2008)
Wikipedia. 2008c. Amoxicillin. Wikipedia, The Free Encyclopedia. Available from http://en.wikipedia.org/wiki/Amoxicillin. (15 April 2008)
Wikipedia. 2008d. Ampicillin. Wikipedia, The Free Encyclopedia. Available from http://en.wikipedia.org/wiki/Ampicillin. (15 April 2008)
Published
2018-10-07
How to Cite
SUMAMPOUW, Oksfriani Jufri. The ANTIBIOTICS SENSITIVITY TEST ON Escherichia coli THAT CAUSE DIARRHEA IN MANADO CITY. JCPS (Journal of Current Pharmaceutical Sciences), [S.l.], v. 2, n. 1, p. 104-110, oct. 2018. ISSN 2598-2095. Available at: <https://journal.umbjm.ac.id/index.php/jcps/article/view/171>. Date accessed: 11 dec. 2018.